Ho'ōla i ka carbon: heʻano o ka adsorbent adsorbenen non-Polar i hoʻohana houʻia. ʻO ka maʻamau, pono ia e holoiʻia me ka maʻi dilited hydroomchlorice, a hahaiʻia e ka ethanol, a laila holoiʻia me ka wai. Ma hope o ka maloʻo ma ka 80 ℃, hiki iā ia ke hoʻohana no ka chrishn chromatography. ʻO Granular i hoʻohālikelikeʻia ka koho maikaʻi loa no ka chromatol chrisn. Inā he mau lau maikaʻi o ka carbon i hanaʻia, pono e hoʻohui i ka nui o ka diaster kūpono e like me ke kōkuaʻana o ka kānana.
ʻO ka carbon i hanaʻia he adsorbdent polar. ʻO kāna Adsorption e kū ana i Silii Gel lāuaʻo Alumina. He ikaika ikaika no nā mea waiwaiʻole. He ikaika ikaika loa ia i ka hopena a me ka nāwaliwali a me ka nāwaliwali ma ka custvent. No laila,ʻo ka hiki ke hoʻopau i ka wai o ka wai ka nāwaliwali a ua ikaika loa ka solivent. Ke hoʻopauʻia ka mea hoʻolaha hoʻolaha mai ka carbon i hanaʻia mai, ua hoʻemiʻia ka polarity, a me ka hiki ke hoʻonuiʻia o ka carbon i ka mea e hiki mai ana. Nā'āpana wai wai, e like me nā amino amino amino a me nā mea kanu a me nā glycosides, ua hoʻokaʻawaleʻia.